Mechanism and kinetic model of microalgal enzymatic hydrolysis for prospective bioethanol conversion

Tetraselmis chuii is a potential microalgae that is in consideration for producing bioethanol owing to its large content of carbohydrates. The glucose production from T. chuii through an enzymatic process with cellulase and xylanase (pretreatment process) and α-amylase and glucoamylase (saccharification process) was studied. The mechanism of the enzymatic process was developed and the kinetic models were then evaluated. For the pretreatment process, enzymes with 30% concentration reacted at 30 °C for 40 min resulted in 35.9% glucose yield. For the saccharification process, the highest glucose yield of 90.03% was obtained using simultaneous α-amylase (0.0006%) and glucoamylase (0.01%) enzymes at 55 °C and for 40 min. The kinetic models fitted well with the experimental data. The model also revealed that the saccharification process performed better than the pretreatment process with a higher kinetic constant and lower activation energy. The proposed kinetic model plays an important role in implementing processes at a larger scale.


Introduction
The increasing rate of population has brought some impacts on many aspects of life, such as the increase in the need for vehicles/transportation and dependence on the industry as a supply of human necessities. These consequently give effect to the increase in the demand for fuel. 1 While, raw material reserves of fossil fuels tend to decrease over time. 2,3 The excessive use of fossil fuels might also cause environmental pollution and climate change. 4 Alternative energy sources of bioethanol, mainly derived from agricultural crops containing starch, such as corn and cassava, have been widely explored. [5][6][7] However, still there are several problems with using crops as raw materials for bioethanol, including the competition in the use of raw materials between food and energy products. 8 Second-generation bioethanol derived from lignocellulosic biomass offers a prospective choice owing to its abundant availability and it does not compete directly with food products. 9,10 However, another problem still exists in relation to the high lignin content that should be removed as it hampers the commercialization of energy products. [11][12][13] As such, microalgae, which does not contain lignin, are available as a source of potentially affordable raw material for bioethanol with a fairly fast growth rate and are even able to grow on critical land, waste, and peat water. [14][15][16] Microalgae have a high carbohydrate content that can be converted into glucose; this material is then easily converted to bioethanol. [17][18][19][20] Microalgae can also absorb CO 2 during the photosynthesis process, thereby reducing the concentration of greenhouse gas emissions in the environment. 21 Tetraselmis chuii is a green microalgal species with a high carbohydrate content of up to 60%. [22][23][24] The process of converting carbohydrates into glucose can be conducted using chemicals or enzymes. [25][26][27] The use of chemicals in this process has a number of advantages, such as affordability and fast processing time. However, some disadvantages of using chemicals are related to the formation of furfural and hydroxymethyl furfural (HMF); those are inhibitors in the further fermentation process of glucose into bioethanol. Furthermore, the formed material also could cause corrosion at high temperatures. Alternatively, the acidic materials should be recovered with high-cost equipment. 28 In contrast, the process of using enzymes, in addition to being able to produce high yields of glucose, can also be carried out at low temperatures without any formation of inhibitors and additional expensive equipment. [29][30][31] The process of converting microalgae into glucose uses an enzyme of a-amylase at the initial stage for the formation of dextrin and is then followed by an enzyme of glucoamylase to produce glucose. 32,33 The main obstacle at this stage is related to the binding of microalgal starch in a rigid cell wall that cannot be in direct contact with the enzyme. 34,35 Therefore, a pretreatment process using cellulase and xylanase enzymes is required. Also, both enzymes are able to break down the microalgae's cell wall and convert cellulose and hemicellulose inside the cell wall into glucose. 36,37 In the next stage, the intracellular starch is converted to dextrin by a-amylase and continued into glucose by glucoamylase. 38,39 The kinetic models are essential to understand an enzymatic process of glucose production using microalgae. This is required for large-scale implementation and operation of the system with the desired standard. 40 The kinetic prediction of an enzymatic process using cellulase and xylanase enzymes to produce glucose has been presented elsewhere; 41 however, the Gompertz model used there could not describe the mechanism inside the enzymatic process in microalgae. The enzymatic kinetics of using glucoamylase and a-amylase with the Michaelis-Menten model was also described elsewhere; 42,43 however, the detailed mechanism with the intermediate step was not considered.
Based on the previous research, the glucose production in the microalgal hydrolysis should be observed in detail based on the steps of the enzymatic process and the types of microalgae. This study aims to optimize the hydrolysis of the microalgae of T. chuii using cellulase and xylanase enzymes (as the pretreatment process) and the hydrolysis of starch microalgae using a-amylase and glucoamylase enzymes (as the saccharication process). The reaction kinetic and the model were also developed in detail to study the mechanism of enzymatic reactions; hence, they can be useful for implementation at an industrial scale.

Microalgal culture and growth media
The microalgae Tetraselmis chuii used in this study were purchased from the Center for Marine Cultivation Development, Lampung Province, Indonesia. The components of microalgae used in this experiment contained 1.07% fat, 19.57% protein, 49.54% hemicellulose, 10.2% cellulose, and 19.62% starch. The cellulase enzyme from Aspergillus niger 22178 was purchased from Sigma-Aldrich, Singapore; the enzyme was in the form of white powder with an activity of 0.8 units per mg solid. Every 0.8 units of cellulase could result in 1.0 mol of glucose from the cellulose substrate at pH 4.0-5.0. Meanwhile, the enzyme endo-1,4-b-xylanase from Trichoderma longibrachiatum X2629 was purchased from Sigma-Aldrich, Singapore in solid form with an activity of 1.0 units per mg solid. Each 1.0 xylanase unit could result in 1.0 mol of glucose per minute from the xylan substrate at pH 4.5 and a temperature of 30°C.

Enzymatic process of microalgae
The process of microalgal pretreatment used two types of enzymes: cellulase (from Aspergillus niger 22178) to convert cellulose into glucose and endo-1,4-b-xylanase (from Trichoderma longibrachiatum X2629) to convert hemicellulose into glucose. Pretreatment experiments using cellulase and xylanase enzymes were carried out by varying the temperature and enzyme concentration. The saccharication process was conducted using the a-amylase enzyme (to produce dextrin) and glucoamylase enzyme (to produce glucose), both of which were obtained from Aspergillus oryzae (with an enzymatic activity of 1.5 units per mg solid) and Aspergillus niger (with an enzymatic activity of 30-60 units per mg solid), respectively. The effect of temperature was also observed in this process.
The rst experiment of the pretreatment process was carried out using 500 mg microalgae put in a 250 mL Erlenmeyer ask and 100 mL of a buffer solution was added (mixture of sodium acetate and acetic acid) with a pH of 4.5 where this pH value was the optimum pH for cellulase and xylanase enzymes from 4.0 to 5.0. Aer the microalgae were dissolved, the Erlenmeyer containing the microalgae was placed into a shaker batch for heating up to 40°C. The enzymes of cellulase and xylanase with the variation of 10%, 20%, or 30% (w/w) were added to the solution by stirring. The pretreatment was carried out for 60 minutes, and samples were taken every 10 minutes. At the end of each pretreatment process, the solution was centrifuged to separate the solution and solids. The pretreatment solution was then heated at 90°C for 15 minutes using a water bath. The sample was further placed into the freezer at −30°C to stop the enzyme activity. To observe the effect of the pretreatment temperature, the enzymatic process with the temperature variation of 40°C, 45°C, 50°C, and 60°C was conducted. For the second experiment of the saccharication process, a similar procedure was followed by utilizing a-amylase and glucoamylase enzymes. The a-amylase concentrations of 0.0002, 0.0006, and 0.001% (w/w) were varied, while the glucoamylase concentrations of 0.002, 0.006, and 0.01% (w/w) were applied. It also included the temperature variation of 45°C, 55°C, 65°C, and 75°C.

Experimental analysis
The contents in microalgae, such as glucose, cellulose, hemicellulose, fat, protein, and starch levels, were analyzed. The cellulose and hemicellulose levels were measured using the Chesson-Datta method. 44 Meanwhile, the analysis of starch content was carried out by acid hydrolysis 45 and the fat content was evaluated through the gravimetric method. 46 The protein content analysis was conducted using the Kjeldahl method. 47 Glucose analysis was carried out using the reducing sugar method of Nelson Somogyi. 48 For the absorbance of the sample solution, the glucose content was determined using a UV-vis spectrophotometer with l (wavelength) of 540 nm; and then the standard curve was plotted based on the calibration results.
Finally, the yield of glucose was calculated based on the following equation: The following equation examined the glucose yield (%): The data were taken in triplicate experiments, and the average data are presented. Statistical analysis was conducted by using ANOVA.

Mechanism and kinetic model of the enzymatic process
The enzymatic kinetic model that explains the phenomena occurring during the enzymatic process was introduced by Michaelis. 49 They assumed that the enzyme directly interacts with the substrate stoichiometrically to form the substrate enzyme, leading to thermodynamic equilibrium. This experiment's enzymatic model is a simultaneous process with cellulose and hemicellulose substrates using cellulase and xylanase enzymes. The enzymatic reaction is described by eqn (3) and (4) for cellulase and xylanase enzymes, respectively.
The kinetic model for the intermediate product (E i S i ) for eqn (3) is described as follows: If the pseudo-steady state hypothesis is assumed, thus, eqn (5) leads to the following: The kinetic model for the substrate (S i ) and the product from eqn (3) is described as follows: A similar step for eqn (4) was applied, and the following equations were obtained: To solve the kinetic model of the enzymatic pretreatment process of cellulose and hemicellulose into glucose, the eqn (6)-(11) were simultaneously applied using MATLAB.
The saccharication process using a-amylase (to produce dextrin) and glucoamylase (to produce glucose) enzymes are described in a model (12): The kinetic model for the rst intermediate product (E i S i ) is described as follows: If the pseudo-steady state hypothesis is assumed, thus, eqn (13) leads to the following: The kinetic model for the substrate (S i ) and the product (dextrin) from eqn (12) is described as follows: The kinetic model for the second intermediate product (E i S i ) is described as follows: If the pseudo-steady state hypothesis is assumed, thus, eqn (17) leads to the following: The equation for the nal product can be obtained as follows: To solve the simultaneous kinetic model of the enzymatic saccharication process of starch into glucose using a-amylase and glucoamylase enzymes, the above equations were simultaneously applied using MATLAB.
To solve the kinetic model of enzymatic pretreatment and the saccharication process above, the ordinary differential equations were solved by using "ODE45" embedded with "fminsearch" to obtain the kinetic constants in MATLAB. The standard error was also examined for each evaluation by the following equation: Results and discussion Enzymatic pretreatment process Fig. 1 shows the kinetic prole of glucose yield for the pretreatment process using individual xylanase and cellulase enzymes at an enzyme concentration of 10% and temperature of 45°C. As shown in the gure, the glucose yield increased with increasing time up to 40 min. The increasing time led to greater enzyme activity. This caused more enzymes binding the substrate to form an enzyme-substrate complex; consequently, more products were formed. [50][51][52] The decrease in glucose yield observed aer 40 min was plausible due to the reduction of the substrate along with time. Conversely, the produced glucose underwent an advanced process in the form of an oxidation process. Glucose was oxidized to form 2 pyruvate with byproducts in the form of 2 NADH and 2 ATP; therefore, as time increased, the pyruvate would also undergo oxidative decarboxylation to form 2 acetyl Co-A. 53 Fig. 2 shows the effect of enzyme concentration on glucose yield for the pretreatment process at 45°C within 40 min. The enzymatic process using the xylanase enzyme was continued by the cellulase enzyme as shown in Fig. 2a, while the simultaneous enzymatic process for both enzymes is shown in Fig. 2b. The higher enzyme concentration led to a higher yield of glucose because the greater enzyme concentration increased enzyme activity; hence, more enzymes would bind to the substrate to form an enzyme-substrate complex and continue to form the product. It was observed that the glucose yield for hemicellulose conversion was smaller than the yield for cellulose. This was because the hemicellulose had the largest component, i.e., xylan, which is a polymer of b (1-4) D-xylopiranose (xylose) with b-1,4-glycoside bonds; [54][55][56] This then caused the branched xylan chains and led to a more complex structure. Moreover, the hemicellulose acted as a glue in every single cell and induced more difficulties for enzymes to form an enzymesubstrate intermediate system.
For a similar condition, the glucose yield obtained from this study was higher than the yield conducted by Harun and Danquah (29.9%). 57 They used the cellulase enzyme of Chlorococum humicola microalgal hydrolysis with the initial pretreatment  process using H 2 SO 4 to improve the yield. It seems that the use of xylanase enzyme in the pretreatment process is more effective than performing acid hydrolysis. As shown in Fig. 2b, the simultaneous use of enzymes was much better than the use of enzymes separately. This was because each enzyme was specic to selectively attack certain substrates as the xylanase enzymes only break down hemicellulose. Though the hemicellulose is the outer cell wall of microalgae compared to cellulose, the breakdown of cellulose directly aer that of hemicellulose was needed; consequently, the simultaneous enzymatic process was more effective. Fig. 3 shows the effect of temperature on glucose yield for the pretreatment process at 30% enzyme concentration for 40 min. The series of enzymatic processes (Fig. 3a) were compared to the simultaneous enzymatic process (Fig. 3b). The glucose yield increased with the increasing temperature as the temperature would affect the molecular kinetic energy of the pretreatment process; thus, leading to the increase in a collision between the substrate and the enzyme molecule. Enzyme activity with the substrate to form the enzyme-substrate complex would increase along with the increase in temperature; therefore, the rate of metabolic processes increased up to the maximum temperature limit. At the conditions above the maximum temperature, the enzyme denaturation process occurred, where the enzyme would no longer function effectively. 58,59 On the other hand, the produced glucose underwent an oxidation process to form pyruvic acid. It was observed that at a temperature of over 45°C the glucose yield for the xylanase enzyme dropped though the yield was relatively constant for cellulase. The same nding was observed for simultaneous processes as a 37.5% yield was obtained with the optimum temperature of 45°C. The optimum temperature obtained from this study is in accordance with the optimum temperature from the other work 58 as for the cellulase enzyme it is in the range 30-45°C and for the xylanase enzyme it is 35-50°C.

Enzymatic saccharication process
The rst step in the enzymatic saccharication of starch in T. chuii was the liquefaction of starch using the enzyme a-amylase that broke a-D-(1-4) glycosidic arbitrarily. a-Amylase is an endo enzyme that works to break a-1,4 glycoside bonds randomly on the inside of amylose and amylopectin. 60,61 The products resulting from the starch saccharication process using the aamylase enzyme were dextrin and the glucoamylase enzyme hydrolyzes a-D (1-4) and a-D (1-6) glycosidic into glucose. 5,32 Both concentration variations were carried out to observe the effect of a-amylase and glucoamylase enzymes on the glucose produced. Fig. 4a and b present the effect of concentration on aamylase enzymes (with 0.01% glucoamylase) and glucoamylase (with 0.0006% a-amylase) on the saccharication of T. chuii starch at 55°C and for 40 min, respectively. As shown in the gure, at the high concentration of a-amylase enzyme (0.0006%), the glucose yield increased to 90.03%. However, at a higher concentration of 0.001%, the yield produced was relatively constant at 90%. The increase in enzyme activity, which continued to increase only up to a concentration of 0.0006%, was due to the decrease in the available substrate; therefore, enzymes that reacted with substrates formed more stable enzyme-substrate compounds. Consequently, adding a higher amount of enzyme did not further affect the enzyme's activity on the substrate. The resulting dextrin, following this step, was converted into glucose by glucoamylase at a constant concentration. As a result, the effect of glucoamylase was relatively the same in this case.
Likewise, in the variation of glucoamylase, as shown in Fig. 4b, when dextrin was formed by a-amylase under constant concentration conditions, increasing the concentration of glucoamylase from 0.002% to 0.01% signicantly increased the yield of glucose from 31% to 90.3%. This indicated that the enzymatic process in the second stage was crucial because quite a lot of dextrin formed must be immediately converted into glucose with the appropriate amount of enzyme is required. If not immediately converted, dextrin would react back to produce complex substrate-enzyme components.
The results of this study are also in line with the research conducted by Choi et al. 32 showing that the concentration of aamylase and glucoamylase enzymes greatly affected the amount of the resulting product. However, they used the enzymes aamylase and glucoamylase separately in each experiment with dextrin and glucose analysis, respectively. This research showed that the optimum concentration of the enzyme a-amylase was 0.005% at the temperature of 90°C within 30 min. In comparison, the optimum glucoamylase was 0.2% at a temperature of 55°C for 30 min as only 23% glucose yield was produced. Although the levels of the glucoamylase enzyme were much higher than those in our work, and the substrate was pure dextrin (no way to form a starch-a-amylase complex), the glucose was still low. The presence of a-amylase that would form a complex compound (starch-a-amylase) helped in controlling the subsequent reaction between glucoamylase and dextrin to produce glucose. Hence, the presence of dextrin, which is not too high, can be converted quickly into glucose. Fig. 5 shows the effect of temperature on glucose yield for the saccharication process using simultaneous a-amylase (0.0006%) and glucoamylase (0.01%) enzymes for 40 min. A sharp increase in glucose yield (3-fold) was obtained from 29% at 45°C to 90.3% at 55°C. However, a signicant drop in yield was observed at temperature aerwards. The increase in enzyme activity towards temperature should increase metabolic processes due to the increased kinetic energy of the reacting molecules, but only up to the optimal temperature limit. A very extreme denaturation process of the enzyme occurred; 62 a drop in yield to 47% was obtained at a temperature of 65°C. However, the glucose yield obtained in our study was found to be higher than that of Megawati et al. 63 In their work, 80% glucose yield was obtained using a-amylase and glucoamylase at a relatively high temperature of 80°C for 5 h; thus, it is certainly less economical. Table 1 presents the hydrolysis yield for various microalgae, enzymes, and operating conditions. The research using individual enzymes showed that the biomass source of Chlamydomonas reinhardtii using the a-amylase enzyme produced the highest yield of 64.2%. 32 Meanwhile, the experiment results of Megawati et al. 63 using simultaneous a-amylase and glucoamylase enzymes were better than those carried out by Phwan et al. 64 with quite high yields, i.e., 80% compared to 35%; even though they used the same microalgal source, i.e. Chlorella. This may be due to the use of 50°C compared to 32°C. As reported, the optimum temperature for using a-amylase and glucoamylase enzymes is in the range of 50-55°C to 55-60°C, respectively. However, our results showed a higher yield of 90%; this could be due to the type of microalgae used, thus affecting the production of glucose.

Statistical analysis
Statistically, in the pretreatment process, the effect of the enzyme concentration for each parallel process and simultaneous process on the glucose yield was insignicantly different at p < 0.05. However, the interaction for both processes was statistically different at p < 0.05. The same trends were observed for the effect of temperature for both processes on glucose yield. For the saccharication process with the effect of enzyme concentration, the use of a-amylase resulted in a signicant difference at p < 0.05 and the use of glucoamylase was, however, statistically insignicant at p < 0.05. Furthermore, the interaction between both enzymes showed a statistically signicant impact at p < 0.05. A similar observation was seen for the effect of temperature on glucose yield for the saccharication process.    6a and b show the kinetic model of the enzymatic pretreatment process using simultaneous cellulose and hemicellulose at various temperatures and enzyme concentrations, respectively. The model well tted the experimental data for all temperatures and concentrations. Although the tting points were less precise at a high temperature of 60°C, the standard error was found still low, i.e., 0.0228. Thus, the proposed kinetic model was developed very well for all temperatures and concentrations with a standard error below 0.03. Table 2 shows the data for kinetic constants of the enzymatic pretreatment process using simultaneous cellulose and hemicellulose at various concentrations. The kinetic constant values for the reaction presented in the table correspond to the kinetic model as shown in Fig. 6. As observed in Table 2, the higher enzyme concentration resulted in a greater rate constant, thus increasing glucose production. The reaction rate constants k 1 and k 5 play an important role in reducing cellulose and hemicellulose substrates, respectively, by producing intermediate enzyme-substrate (ES) products. The greater value of k 1 and k 5 led to the faster reduction of cellulose and hemicellulose concentration to produce the intermediate products. On the other hand, the reaction rate constants of k 3 and k 7 played a signicant role in changing intermediate compounds to produce glucose. It means that the higher values of k 3 and k 7 contributed to the faster decrease in the intermediate (ES) component to produce glucose.
It was also observed that the value of k 1 was higher than k 5, indicating that the cellulose conversion rate into intermediates was faster than the rate of hemicellulose into that one. However,    Table 2, the values of k 1 and k 5 were found smaller than the values of k 3 and k 7 ; indicating that the rate of glucose production was slower than the rate of formation of intermediate compounds (ES). Therefore, this conrmed that glucose production became the determining step for the reaction rate as the slowest step in the kinetic model was observed. 67-69 Table 3 shows the data for kinetic constants of the enzymatic pretreatment process using simultaneous cellulose and hemicellulose at various temperatures. As shown in the table, the value of k increased with increasing temperature, but the kinetic constant decreased at 50°C and even more at 60°C. This nding conrmed the previous results that were related to the denaturation process occurring at a temperature of 50°C for both cellulase and xylanase enzymes, thereby reducing the effectiveness of the enzyme. As reported, the cellulase enzyme has an optimum range temperature of 45-50°C, and the denaturation process occurred at a later temperature; 62,70 while the xylanase enzyme had an optimum range temperature of 50-55°C and further higher temperature conrmed the denaturation process. 71,72 However, as shown in Table 3, the k 5 value, relatively, did not have signicant changes at all temperatures, and this indicated that the conversion process of hemicellulose substrates into intermediate compounds (ES) proceeded well, and the process did not experience any denaturation. Another possibility is that the increase in temperature impacted the conversion process of intermediate compounds from hemicellulose enzymes to glucose. In addition, the nding data was differently observed for the kinetic cellulase rate as the allkinetic constants decreased, thus indicating the denaturation effect to be inuential on the cellulase enzyme. Fig. 7 shows the kinetic model of the enzymatic saccharication process using a-amylase and glucoamylase enzymes at various temperatures. The kinetic model excellently tted the experimental data for all temperatures. Again, the proposed kinetic model for the enzymatic saccharication process has been generated very well with a standard error below 0.05. This kinetic development is useful for the implementation process at industrial scale. Table 4 shows the kinetic constants of the enzymatic saccharication process using simultaneous aamylase and glucoamylase enzymes. As shown in Table 4, the greatest kinetic constant was obtained at 55°C. The higher temperature should increase the reaction rate constant, but at 65°C, the reaction rate constants decreased, indicating that the enzyme started to experience denaturation. As reported, 73-75 the a-amylase enzyme has an optimum range temperature of 50-55°C, and the denaturation process occurs at a temperature of 70°C. Meanwhile, glucoamylase enzyme has an optimum range temperature of 55-60°C, and the enzyme with the process at a temperature of 70°C was observed to be denaturation. [76][77][78]   As presented in Table 4, the value of k 11 was higher than that of k 9 , indicating that the process of forming dextrin (P1) was more inuential than the conversion of starch into a complex compound or intermediate 1 (ES1). The value of k 13 was greater than that of k 9 , indicating that the conversion rate of dextrin to intermediate 1 (ES1) was more dominant than the rate of conversion of starch to intermediate 2 (ES2). However, the k 14 value was also quite large, which indicated that the reversible rate of intermediate 2 into dextrin was also quite signicant. On the other hand, the higher value of k 11 compared to the k 15 indicated that the dextrin production rate was higher than the glucose production rate from each intermediate (ES1 and ES2, respectively). This might be because the reaction was simultaneous; hence, the rapid production of dextrin was directly bound by the glucoamylase enzyme to be converted into glucose, even though it went through the stages of the dextringlucoamylase (ES2) complex compound. It then conrmed that the rate of glucose production is the determining step of the reaction rate.
In this study, the effect of temperature was also observed using the Arrhenius equation. The calculated parameter was the activation energy (E a ), as shown in Table 5. The activation energy for the series of pretreatment processes from cellulose to glucose followed by hemicellulose to glucose had a higher value (31.8 and 30.37 kJ mol −1 ) compared to the one for the simultaneous pretreatment process (18.21 kJ mol −1 ). This indicated that the reaction rate of glucose formation in the simultaneous process was faster than in the series process, as a larger value of k was observed. Also, the glucose production for the simultaneous pretreatment process from cellulose and hemicellulose had a higher activation energy value (18.21 kJ mol −1 ) than the production for the simultaneous saccharication process (11.29 kJ mol −1 ). Thus, the reaction rate for the saccharication process was also faster than the rate for the pretreatment process, as indicated by the greater k value. This study's results are aligned with the research conducted by Harun and Danquah 57 in which a smaller activation energy led to a faster reaction speed.
As shown in Table 5, the value of E a in the pretreatment process was small, while the value of k became larger at higher temperatures. This is according to the Arrhenius equation 0 @ k ¼ Ae À E RT 1 A that the higher temperature leads to a smaller negative exponent value in the Arrhenius equation; hence, the greater value of the reaction rate constant (k) is obtained. This consequences in a faster reaction rate. If the temperature of the pretreatment process is too high, it will cause the enzyme to deactivate more quickly. As previously observed in this study, the value of the reaction rate constant decreased at temperatures above 45°C. In addition, the use of simultaneous enzymes and the increase in the concentration of enzymes led to a smaller value of E a , thus resulting in a larger value of k. It was reasonable because when more enzymes are used, more possibility for the substrate to form an enzyme-substrate complex, thereby accelerating the reaction. As reported, 79 an increase in the enzyme in the substrate increased to the dextrose equivalent, indicating a faster reaction.

Conclusions
The pretreatment process using cellulase and xylanase enzymes and the saccharication processes using a-amylase and glucoamylase enzymes were successfully carried out to produce high glucose yield from Tetraselmis chuii microalgae. Increasing enzyme concentration increased the glucose yield, while the yield increased with the temperature. The enzymatic hydrolysis of the pretreatment process with an enzyme concentration of 30% at 30°C resulted in a glucose yield of 35.9%. The hydrolysis reaction of the saccharication process conducted at 55°C and pH of 4.5 resulted in a glucose yield of 90.03%. The proposed kinetic model well tted with the experimental data for both pretreatment and saccharication processes. The kinetic studies revealed that in the pretreatment process, the denaturation process was observed at the conversion of the intermediate compounds from hemicellulose enzymes to glucose. Furthermore, in the saccharication process, the rate of glucose production became the determining step of the reaction rate as the dextrin formation rate and intermediate process underwent rapidly. The faster rate of the saccharication process was observed compared to the rate of the pretreatment process as indicated by the activation energy of 11.29 kJ mol −1 and 18.21 kJ mol −1 , respectively. The kinetic study showed that the developed kinetic model could be a potential base for implementation at the industrial scale.

Conflicts of interest
There are no conicts to declare.